Communicate with your colleagues about antibodies and critical laboratory protocol issues. Antibodies are the hot items being sought, but you can query—and help out—other researchers on questions of methodology or other research reagents and tools. Send us your postings and comments and we will feature them in the "What's New" section of the website, and we'll send them out in the SRF Newsletter. Responses will be posted to this page. Please let us know if you have any success through this feature of the website.

A great resource for information on commercially available antibodies is the encyclopedic Alzheimer Research Forum Antibody Directory. More antibodies than you can shake a pipette at!

Q. Hi, I am searching for an antibody against Br-cadherin (also called cadherin 12 or neuronal cadherin-2). This antibody should be suitable for immunohistochemistry and react on human tissue. Thanks, Mihovil Mladinov—Posted 23 March 2007

Q. Hi! I am trying to block Cdk5 expression in primary neuronal cultures by RNA intereference, using Dharmacon smart pools and oligofectamine but without great success. Does anybody have any suggestions/protocols??? Thank you, Xenia Antoniou—Posted 22 March 2007

Q. I am desperately seeking a DYRK1a antibody that can IP and immunodeplete DYRK1a from rat brain high speed centrifuged supernatant. Ideally an antibody that does NOT cross react with anything else in rat. What concentration/conditions would you use to for best immunodepletion results? Reference: Kentrup et al., 1996. Thanks—Shen, King's College, London—Posted 4 January 2007

Q. Hi! I'm desperately looking for the cell line named 7PA2 (CHO cells), and I would be very grateful if you could either provide me with a vial or if you'd know where to purchase them. Cheers.—Barbara Bennani-Baiti (barbara.bennani-baiti@univie.ac.at)—Posted 30 October 2006

Q. I am currently seeking antibodies that will recognize mouse VEGF isoforms (188,164, and 120) on a Western blot. Any information will be greatly appreciated. I have tried a couple of different antibodies to date and can only see 164. Thanks.—Sheila Harris (sheila.harris@sheffield.ac.uk)—Posted 22 September 2006

Q. Hi, I am about to complete a set of experiments for which I need anti-phosphoserine 880 GluR2 antibody. This antibody is not commercially available anymore. Has anybody got any? Thank you very much.—Elsa Martinez (elsmar@neuro.mpg.de)—Posted 5 December 2005

Q. I'm looking for an antibody that will on Western Blot pick up neurofilament M in mouse tissue that is specific for the phosphorylated form of the protein. If you are aware of some place I could purchase this antibody, please reply. Thanks!—Anita Gnezda (agnezda@iupui.edu) Department of Pathology, IUSOM, indianapolis Indiana—Posted 30 September 2005

Q. I'm interested in working with the IGFBP3 antibody from Gene Tex (product id = 16328), but I need some more information about how/ if it works in Western blotting and eventually immunoprecipitation. Has anybody already used it and could ensure me that it really works? Thanks a lot!—Heide Vogel (Heide.Vogel@ukmuenster.de) Institute for Neuropathology, Munster, Germany—Posted 31 August 2005

Q. I am using OBTOO30-Monoclonal Rat -anti-BrdU (as primary antibody) on mouse brain. The pregnant mums or animals were injected with Brdu in vivo and then their embryos were processed for paraffin. I am not getting any results and I think this is because of the primary antibody (OBT0030). Is it the right antibody for this kind of experiment? Thanks!—Noura (xxnalmin@uq.edu.au)—Posted 23 August 2005